Immunomodulation effect of pmethaxy cinnamat ethyl ester kencur compound (Kaempferia galanga Linn) to the phagocytosis viability in vitro

Revilla, Gusti and Yerizel, Eti (2001) Immunomodulation effect of pmethaxy cinnamat ethyl ester kencur compound (Kaempferia galanga Linn) to the phagocytosis viability in vitro.

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Abstract

Immunomodulation effects of aquaous and methanol extracts of kencur (Kaempferia galanga Linn) were studied for phagocytic effect in vitro. Methanol extract of kencur was also studied for phagocytic effect in vivo. It is known that methanol extract of kencur has stronger suppressive effect than aquaous one. This is probably due to pmethoxy cinnamat ethyl ester contained within methanol extracts of kencur. The aim of this study was to investigate the immunosuppressive effect of pmethoxy cinnamat ethyl ester in kencur on phagocytosis in vitro. For this purpose an in vitro granulocyte phagocytosis assay was used (Wagner, et al. 1991). This method assesses the speed of neuthrophyl ingestion on antigen after treated with test substance. The source of neutrophil was taken from five healthy adults and then separated from other cells using ficoll. They were divided into experimental and control group. Experimental group was further divided into three subgroups receiving of 100 ,ug/ml, 150 ,ug/ml and 200 uglml pmethoxy cinnamat ethyl ester. Control group received the solvent compound of pmethoxy cinnamat ethyl ester. Phagocytic effect can be seen from the incubating result of neuthrophyl with yeast cells (Sacchromyces cereviceae), to enable us to count phagocytosis index (PI) and the phagocytic percentages (PP). The PP of neuthrophyls treated with pmethoxy cinnamat ethyl ester were  7.826% of 100 (g/ml and 19.909 for 200 (g/ml concentration respectively. The negative value showed that pmethoxy cinnamat ethyl ester compound has immunosuppressive effect on the phagocytosis capability of neuthropyl in vitro. Statistically, the,phagocytic effect was significantly reduced by increasing concentration of pmethoxy cinnamat ethyl ester (p

Item Type: Article
Subjects: Collections > Koleksi Perpustakaan Di Indonesia > Perpustakaan Di Indonesia > JKPTYARSI > Research Report > Kedokteran
Divisions: Universitas Komputer Indonesia > Perpustakaan UNIKOM
Depositing User: M.Kom Taryana Suryana
Date Deposited: 16 Nov 2016 07:37
Last Modified: 16 Nov 2016 07:37
URI: https://repository.unikom.ac.id/id/eprint/2434

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