Jujur Wibawa, Pratama (2012) Konstruksi pustaka genom Bacillus sp.BAC4 menggunakan vektor pHB201 dan sel inang E.coli JM101.
Full text not available from this repository.Abstract
The genomic library of a local strain of Bacillus sp. BAC4 had been constructed in pHB201 shuttle vector employing E. coli JM101 as a host cell. The 3-10 kb chromosomal DNA fragments of the Bacillus sp. BAC4, obtained from digestion with BamHI enzyme, were ligated into undephosphorilated pHB201 vector linearized by the same enzyme. The ligation reaction was catalyzed by T4 DNA ligase. The resulted recombinant plasmids were used to transform E. coli JM101 cells which had been made competent by means of CaCl2 solution. A total of 1500 transformant cells were successfully isolated from the LB media containing erythromycin, X-Gal, and IPTG. This process gave a transformation efficiency of 7.5 x 1.000.000 colonies/ug DNA pHB201 and a recombination frequency of about 75 %. BamHI digestion analysis on 11 recombinants randomly selected from 31 clones indicated that the probability of obtaining independent clones was only about 6.5 %. These independent clones contained a DNA insert of 5.58 to 8.25 kb in size.
| Item Type: | Article |
|---|---|
| Subjects: | Collections > Koleksi Perpustakaan Di Indonesia > Perpustakaan Di Indonesia > JBPTITBPP > S2-Theses > Natural Sciences > Chemistry > 1999 |
| Divisions: | Universitas Komputer Indonesia > Perpustakaan UNIKOM |
| Depositing User: | Admin Repository |
| Date Deposited: | 16 Nov 2016 07:37 |
| Last Modified: | 16 Nov 2016 07:37 |
| URI: | http://repository.unikom.ac.id/id/eprint/2752 |
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