Comparison using three different discontinuous pencof gradients in human sperm preparation

Waeito, Bambang (2002) Comparison using three different discontinuous pencof gradients in human sperm preparation.

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Abstract

To carry out an Assisted Reproductive Technology (ART), a sperm preparation is needed. There are a variety of sperm preparation available alo: washing and swim-up, albumin collumn filtration, glass wool filtration and discontinuous percoll gradient. Method that recommended by WHO is two discontinuous percoll gradient (40% and 80'1). The aim of this study is to know the effectiveness of additional of percoll gradient to that of two discontinuous percoll gradient. The sperm was taken from 30 males who came to Andrology laboratory of Research and Development Center of Reproduction Health Service (Puslitbang Yankes) Surabaya for regular infertility consultation during January 1 - March 1, 1998 period. Sample will be taken if the sperm volume > 2,5 ml, ranged from extreme oligo-asthenoteratozoospermia to normo-spermia. Samples were divided into 3 groups of 10 each, where each group was treated by two (40'0 and 90Yq), three (4000 60% and 90%) and six (40'16, 60%, 70'16, 80%, 90% and 100%) discontinuous percoll gradient. Parameters of the post treated sperm are sperm concentration, sperm motility of type (a b) and sperm form. The results show that there was a significant difference in sperm concentration among the three discontinuous percoll gradient. In the six discontinuous percoll gradients, sperm concentration was highly significant lowered (p=0, 0100). There was also a significant difference of sperm motility of type (a b) before and after sperm preparation using two (p=0, 0021) and three discontinuous percoll gradients (p=0, 0073). It can be concluded that any addition to two discontinuous percoll gradient was proved to be useless.

Item Type: Article
Subjects: Collections > Koleksi Perpustakaan Di Indonesia > Perpustakaan Di Indonesia > JKPTYARSI > Journal > Kedokteran > Jurnal Kedokteran YARSI > Tahun 2000
Divisions: Universitas Komputer Indonesia > Perpustakaan UNIKOM
Depositing User: M.Kom Taryana Suryana
Date Deposited: 16 Nov 2016 07:38
Last Modified: 16 Nov 2016 07:38
URI: https://repository.unikom.ac.id/id/eprint/3331

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